Truncated WT1 Protein Isoform Expression Is Increased in MCF-7 Cells with Long-Term Estrogen Depletion

Figure 2

Expression of WT1 in MCF-7 cells cultured with estrogen depletion. (a) The 36-38 kDa and 52-54 kDa WT1 isoform expression in MCF-7 cells in depletion of estrogen in a short term (hours). (b) The 36-38 kDa and 52-54 kDa WT1 isoform expression in MCF-7 cells following the long-term depletion of estrogen (until 27 weeks). In both figures, β-actin expression was used as the endogenous control. (c, d) Show the densitometry analysis of the relative expression of WT1 isoforms. The 52-54 kDa isoform is shown in white columns and the 36-38 kDa isoform in black columns.

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