Sunitinib Combined with Th1 Cytokines Potentiates Apoptosis in Human Breast Cancer Cells and Suppresses Tumor Growth in a Murine Model of HER-2^pos Breast Cancer

Figure 6

Sunitinib suppresses interferon-gamma secretion by stimulated T cells. (a) Example IFN-γ ELISPOT wells from individual healthy donor PBMCs stimulated with a mixture of common viral peptide recall antigens (left panel) or tetanus toxoid (right panel) in the presence or absence of sunitinib (“sunit”; 10 μM). (b) Composite analysis of each of the 3 donors for viral recall peptides (left panel) and 3 donors for tetanus toxoid (right panel). Statistical analysis by one-way ANOVA indicated a significant difference between sunitinib-treated and sunitinib-untreated groups. (c) IFN-γ ELISA analysis of 72-hour culture supernatants from 8 unique allogeneic MLRs (A–H) where activated dendritic cells (DC) and lymphocyte-rich elutriation fractions were cocultured at 1 : 40 stimulator : responder ratios in the presence or absence of 10 μM sunitinib.

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