Research Article
Association of XRCC3, XRCC4, BAX, and BCL-2 Polymorphisms with the Risk of Breast Cancer
Table 3
PCR and RFLP procedures and expected products of XRCC3 Thr241Met, XRCC4 G(‐1394) T, Bax G(-248) A, and BCL2 C(–938) A genes.
| | Genes | Primers (forward and reverse) | PCR product | Restriction enzyme | Restriction products |
| | XRCC3 Thr241Met | 5-GGTCGAGTGACAGTCCAAAC-3
5-TGCAACGGCTGAGGGTCTT-3 | 456 bp | Nla III (37°C) | Thr/Thr: bp
Met/Met: bp | | XRCC4 G(‐1394)T | 5-AGAAGGGCAATCCACCTTTG-3
5-AGCATTAGCGCTTCTCGAG-3 | 257 bp | Mbo II (37°C) | GG: bp
TT: 257 bp | | Bax G(-248)A | 5-CATTAGAGCTGCGATTGGACCG-3
5-GCTCCCTCGGGAGGTTTGGT-3 | 109 bp | Msp I (37°C) | GG: bp
AA: 109 bp | | BCL2 C(‐938)A | 5-CTGCCTTCATTTATCCAGCA-3
5-GGCGGCAGATGAATTACAA-3 | 262 bp | Bcc I (37°C) | CC: bp
AA: 262 bp |
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