In Vitro Growth Inhibition, Caspase-Dependent Apoptosis, and S and G2/M Phase Arrest in Breast Cancer Cells Induced by Fluorine-Incorporated Gold I Compound, Ph3PAu[SC(OMe)=NC6H4F-3]

Figure 1

Viability percentage of cells by MTT assays. (a–c) MDA-MB-231 cells treated by the gold compound 3F1, 3F3, and 3FL. (d–f) MCF-7 cells treated by the gold compound 3F1, 3F3, and 3FL. (g) H9c2 cells treated by the gold compound 3F1. (h, i) Dose-response curves to determine the IC₅₀ of the compound 3F1. Cells were treated with various concentrations of 3F1, 3F3, and 3FL compound for 24 h. Cells in the control wells were treated with 0.1% DMSO. MDA-MB-231, MCF-7, and H9c2 cells in positive control wells were treated with cisplatin (cis-diamineplatinum (II) dichloride, CDDP) at 50 μM, 33 μM, and 236 μM, respectively. Data are presented as the of three independent experiments performed in triplicate. Statistical significance was analyzed by one-way ANOVA and a post hoc Dunnett’s multiple comparison test. was considered significantly different from the control.

(a) MDA-MB-231 treated with 3F1

(b) MDA-MB-231 treated with 3F3

(c) MDA-MB-231 treated with 3FL

(d) MCF-7 treated with 3F1

(e) MCF-7 treated with 3F3

(f) MCF-7 treated with 3FL

(g) H9c2 treated with 3F1

(h) MDA-MB-231 treated with 3F1

(i) MCF-7 treated with 3F1