In Vitro Growth Inhibition, Caspase-Dependent Apoptosis, and S and G2/M Phase Arrest in Breast Cancer Cells Induced by Fluorine-Incorporated Gold I Compound, Ph3PAu[SC(OMe)=NC6H4F-3]

Figure 6

Caspase-3/-7-dependent apoptosis in cells treated with compound 3F1 were detected using a MUSE flow cytometer. MDA-MB-231 (a) and MCF-7 (b) cells were treated for 24 h with the respective IC₅₀ dose of the novel gold-based compound 3F1 or with the respective IC₅₀ dose of cisplatin (CDDP) for positive control samples or with 0.1% DMSO for the negative controls. Apoptotic cells with activated caspase-3/-7 are plotted in the upper and lower right quadrants. Viable cells are plotted in the left lower quadrant, whereas necrotic cells are shown in the left upper quadrant of the representative scattered plot.

(a) MDA-MB-231

(b) MCF-7