International Journal of Biomedical Imaging

International Journal of Biomedical Imaging / 2006 / Article

Open Access

Volume 2006 |Article ID 93438 | 6 pages |

High-Speed Fluorescence Imaging and Intensity Profiling of Femtosecond-Induced Calcium Transients

Academic Editor: Vasilis Ntziachristos
Received21 Sep 2005
Revised14 Dec 2005
Accepted05 Jan 2006
Published07 Mar 2006


We have demonstrated a combined imaging system, where the physiology of biological specimens can be imaged and profiled at 10–20 frames per second whilst undergoing femtosecond laser irradiation. Individual GH3 cells labeled with the calcium fluorophore Fluo-3 were stimulated using a counter-propagating focused femtosecond beam with respect to the imaging system. As a result of the stimulation, calcium waves can be generated in COS cells, and laser-induced calcium oscillations are initiated in the GH3 cells. Single-photon fluorescence images and intensity profiles of the targeted specimens are sampled in real-time using a modified PerkinElmer UltraView LCI microscope.


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Copyright © 2006 Daniel Day et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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