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International Journal of Biomedical Imaging
Volume 2007 (2007), Article ID 79710, 9 pages
Research Article

Development of a Confocal Optical System Design for Molecular Imaging Applications of Biochip

1Medical Systems Biology Research Center, School of Medicine, Tsinghua University, Beijing 100084, China
2National Engineering Research Center for Beijing Biochip Technology, Beijing 102206, China
3Department of Precision Instruments and Mechanology, School of Mechanical Engineering, Tsinghua University, Beijing 100084, China

Received 19 April 2007; Revised 23 June 2007; Accepted 15 July 2007

Academic Editor: Jie Tian

Copyright © 2007 Guoliang Huang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


A novel confocal optical system design and a dual laser confocal scanner have been developed to meet the requirements of highly sensitive detection of biomolecules on microarray chips, which is characterized by a long working distance (wd>3.0mm), high numerical aperture (NA=0.72), and only 3 materials and 7 lenses used. This confocal optical system has a high scanning resolution, an excellent contrast and signal-to-noise ratio, and an efficiency of collected fluorescence of more than 2-fold better than that of other commercial confocal biochip scanners. The scanner is as equally good for the molecular imaging detection of enclosed biochips as for the detection of biological samples on a slide surface covered with a cover-slip glass. Some applications of gene and protein imagings using the dual laser confocal scanner are described.