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International Journal of Biomedical Imaging
Volume 2010, Article ID 105984, 11 pages
Research Article

Multiscale Characterization of the PEPCK- Mouse through 3D Cryoimaging

1Department of Biomedical Engineering, Case Western Reserve University, Cleveland, OH 44106, USA
2Department of Biochemistry, Case Western Reserve University, Cleveland, OH 44106, USA
3Department of Radiology, University Hospitals of Cleveland, Cleveland, OH 44106, USA

Received 20 November 2009; Accepted 24 February 2010

Academic Editor: Haim Azhari

Copyright © 2010 Debashish Roy et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


We have developed, for the Case 3D Cryo-imaging system, a specialized, multiscale visualization scheme which provides color-rich volume rendering and multiplanar reformatting enabling one to visualize an entire mouse and zoom in to organ, tissue, and microscopic scales. With this system, we have anatomically characterized, in 3D, from whole animal to tissue level, a transgenic mouse and compared it with its control. The transgenic mouse overexpresses the cytosolic form of phosphoenolpyruvate carboxykinase (PEPCK-C) in its skeletal muscle and is capable of greatly enhanced physical endurance and has a longer life-span and reproductive life as compared to control animals. We semiautomatically analyzed selected organs such as kidney, heart, adrenal gland, spleen, and ovaries and found comparatively enlarged heart, much less visceral, subcutaneous, and pericardial adipose tissue, and higher tibia-to-femur ratio in the transgenic animal. Microscopically, individual skeletal muscle fibers, fine mesenteric blood vessels, and intestinal villi, among others, were clearly seen.