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International Journal of Biomedical Imaging
Volume 2010 (2010), Article ID 654506, 12 pages
Research Article

Accurate Coregistration between Ultra-High-Resolution Micro-SPECT and Circular Cone-Beam Micro-CT Scanners

1Image Sciences Institute and Rudolf Magnus Institute, University Medical Center Utrecht, Universiteitsweg 100, 3584 CG Utrecht, The Netherlands
2Physics Department, Peking University, Beijing 100871, China
3MILabs B.V., Heidelberglaan 100, 3584 CX Utrecht, The Netherlands
4Department of Radiation, Radionuclides, and Reactors, Section Radiation Detection and Medical imaging, Delft University of Technology, Mekelweg 15, 2629 JB Delft, The Netherlands

Received 15 April 2010; Revised 6 July 2010; Accepted 31 July 2010

Academic Editor: Habib Zaidi

Copyright © 2010 Changguo Ji et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Introduction. Spatially registering SPECT with CT makes it possible to anatomically localize SPECT tracers. In this study, an accurate method for the coregistration of ultra-high-resolution SPECT volumes and multiple cone-beam CT volumes is developed and validated, which does not require markers during animal scanning. Methods. Transferable animal beds were developed with an accurate mounting interface. Simple calibration phantoms make it possible to obtain both the spatial transformation matrix for stitching multiple CT scans of different parts of the animal and to register SPECT and CT. The spatial transformation for image coregistration is calculated once using Horn's matching algorithm. Animal images can then be coregistered without using markers. Results. For mouse-sized objects, average coregistration errors between SPECT and CT in X, Y, and Z directions are within 0.04 mm, 0.10 mm, and 0.19 mm, respectively. For rat-sized objects, these numbers are 0.22 mm, 0.14 mm, and 0.28 mm. Average 3D coregistration errors were within 0.24 mm and 0.42 mm for mouse and rat imaging, respectively. Conclusion. Extending the field-of-view of cone-beam CT by stitching is improved by prior registration of the CT volumes. The accuracy of registration between SPECT and CT is typically better than the image resolution of current ultra-high-resolution SPECT.