International Journal of Biomedical Imaging / 2011 / Article / Tab 3

Research Article

Estimating Cell Count and Distribution in Labeled Histological Samples Using Incremental Cell Search

Table 3

Count results of ICS and ITCN at different resolutions.

ResolutionsManual countsITCNICS
Cell diameter DAB (pixels)Cell diameter DAPI (pixels)Corresponding optical magnificationBrightfield (DAB stained)UV (DAPI stained)Brightfield (DAB stained)UV (DAPI stained)Brightfield (DAB stained)UV (DAPI-stained)
ThresholdAverage countThresholdAverage countAverage countaverage count

4532.4400xN/AN/A0.133.20306.626.5376.4
3223200x26.0411.50.233.60.1261.026.2375.3
2516.2100xN/AN/A0.338.00.1269.026.2374.6
1611.550xN/AN/A0.836.70.1288.727.7376.6
85.7512.5xN/AN/A250.00.4316.027.7367.0

Average across resolutions:26.0411.538.3288.326.8374.0

Coefficient of variation across resolutions:14.2%16.3%19.3%10.9%8.9%2.7%

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