Estimating Cell Count and Distribution in Labeled Histological Samples Using Incremental Cell Search

Table 3

Count results of ICS and ITCN at different resolutions.

Resolutions			Manual counts		ITCN				ICS
Cell diameter DAB (pixels)	Cell diameter DAPI (pixels)	Corresponding optical magnification	Brightfield (DAB stained)	UV (DAPI stained)	Brightfield (DAB stained)		UV (DAPI stained)		Brightfield (DAB stained)	UV (DAPI-stained)
					Threshold	Average count	Threshold	Average count	Average count	average count
45	32.4	400x	N/A	N/A	0.1	33.2	0	306.6	26.5	376.4
32	23	200x	26.0	411.5	0.2	33.6	0.1	261.0	26.2	375.3
25	16.2	100x	N/A	N/A	0.3	38.0	0.1	269.0	26.2	374.6
16	11.5	50x	N/A	N/A	0.8	36.7	0.1	288.7	27.7	376.6
8	5.75	12.5x	N/A	N/A	2	50.0	0.4	316.0	27.7	367.0
Average across resolutions:			26.0	411.5		38.3		288.3	26.8	374.0
Coefficient of variation across resolutions:			14.2%	16.3%		19.3%		10.9%	8.9%	2.7%