Detection and Quantification of Fluorescent Cell Clusters in Cryo-Imaging
Figure 1
The appearance of single cells/microspheres is distorted by pixelation. The shape greatly depends upon the location of the microsphere with respect to the sampling grid of the CCD camera. Three separate single microspheres in OCT are shown with their corresponding integrated intensity. Although the images appear much different, the integrated intensity is nearly equal and within the 5% variation specified by the manufacturer.
