Space and Time Resolved Detection of Platelet Activation and von Willebrand Factor Conformational Changes in Deep Suspensions

Figure 8

Enhanced signals and signal tracks of (a) globular rVWF-eGFP; (b) aggregates (top) and stretched filaments (bottom). Tracks are composed superimposing several occurrences of the same signal at a fixed time interval. Variation in the enhanced signal strength among different images is due to different levels of background noise and signal intensity in the raw images. (c) Number of distinct rVWF-eGFP multimers observed before (left) and after (right) application of shear in samples S1–S4. A substantial increase in the number of detected multimers after application of shear stress is observed in all cases. E: EDTA; A: ADAMTS13; ± indicates the presence/absence of the two compounds.

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