Three-Dimensional Imaging of Pulmonary Fibrotic Foci at the Alveolar Scale Using Tissue-Clearing Treatment with Staining Techniques of Extracellular Matrix

<div>Fluorescence labeling of fibrotic foci in the lungs using Col-F. (a) Lung sections were stained using Col-F. Green fluorescence indicates the localization of Col-F. Scale bar is 100 μm. (b–d) Fibrotic lungs were perfusion-fixed, excised, and stained using Col-F. After ClearT2 tissue-clearing treatment, the lungs were observed using confocal microscopy. (b) Three-dimensional images were reconstructed from single-plane images. Each 5 μm stack is 20–120 μm below the lung surface. (c) The image of cross-sections indicated by the red lines in (b). (d) Single-plane images at the indicated depth (μm) from the lung surface. Green fluorescence indicates the localization of Col-F. The scale bar is 100 μm.</div>

International Journal of Biomedical Imaging

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Figure 6

Figure 6: Three-Dimensional Imaging of Pulmonary Fibrotic Foci at the Alveolar Scale Using Tissue-Clearing Treatment with Staining Techniques of Extracellular Matrix 

Figure 6 | Three-Dimensional Imaging of Pulmonary Fibrotic Foci at the Alveolar Scale Using Tissue-Clearing Treatment with Staining Techniques of Extracellular Matrix 