Three-Dimensional Imaging of Pulmonary Fibrotic Foci at the Alveolar Scale Using Tissue-Clearing Treatment with Staining Techniques of Extracellular Matrix

<div>Quantitative analysis of fluorescence-labeled fibrotic foci in the lungs using Col-F after Clear<sup>T2</sup> tissue-clearing treatment. (a) Hydroxyproline content in fibrotic mouse lungs. (b) Blue coloration indicating extracellular matrix localization on the lung tissue section. (c) Fluorescent intensities derived from single-plane images of Col-F at the indicated depth (<i>μ</i>m) from the lung surface. 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International Journal of Biomedical Imaging

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Figure 7

Figure 7: Three-Dimensional Imaging of Pulmonary Fibrotic Foci at the Alveolar Scale Using Tissue-Clearing Treatment with Staining Techniques of Extracellular Matrix 

Figure 7 | Three-Dimensional Imaging of Pulmonary Fibrotic Foci at the Alveolar Scale Using Tissue-Clearing Treatment with Staining Techniques of Extracellular Matrix 