A Biomimetic Approach for the Creation of Two-Dimensional Microscale Surface Patterns: Creation of Isolated Immunological Synapses
Figure 4
Fluorescent micrographs showing bound anti-LFA-1-FITC ((a), (d); GFP filter), bound anti-CD3ε-AlexaFluor568 ((b), (e); TRITC filter), and an overlay of both channels ((c), (f)). The white scale bars represent 5 m. The images are from two representative patterns of different dimensions fabricated in parallel on the same chip. Experiments were repeated three times with similar results.