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International Journal of Biomaterials
Volume 2011, Article ID 109638, 11 pages
http://dx.doi.org/10.1155/2011/109638
Research Article

In Vivo and In Vitro Study of a Polylactide-Fiber-Reinforced β-Tricalcium Phosphate Composite Cage in an Ovine Anterior Cervical Intercorporal Fusion Model

1Neurosurgical Unit, Department of Surgery, Turku University Central Hospital, P.O. Box 52, 20521 Turku, Finland
2Institute of Biomedical Technology, University of Tampere, 33014 Tampere, Finland
3Science Center, Tampere University Hospital, P.O. Box 2000, 33521 Tampere, Finland
4Bioretec Ltd., Hermiankatu 22, 33720 Tampere, Finland
5Tampere School of Public Health, University of Tampere, 33014 Tampere, Finland
6Department of Plastic Surgery, Tampere University Hospital, P.O. Box 2000, 33521 Tampere, Finland
7Department of Oral and Maxillofacial Surgery, University of Oulu, P.O. Box 5000, 90014 Oulu, Finland
8Department of Anaesthesiology, Intensive Care, Emergency Care and Pain Medicine, Turku University Central Hospital, P.O. Box 52, 20521 Turku, Finland
9Department of Pathology, Turku University Central Hospital, P.O. Box 52, 20521 Turku, Finland
10Department of Radiology, Turku University Central Hospital, P.O. Box 52, 20521 Turku, Finland
11Department of Eye, Ear, and Oral Diseases, Tampere University Hospital, P.O. Box 2000, 33521 Tampere, Finland
12Department of Biomedical Engineering, Tampere University of Technology, P.O. Box 692, 33101 Tampere, Finland
13Orthopedic Research Unit, Department of Orthopedic Surgery and Traumatology, University of Turku, Lemminkäisenkatu 2, 20520 Turku, Finland

Received 16 March 2011; Accepted 20 August 2011

Academic Editor: Claudio Migliaresi

Copyright © 2011 Janek Frantzén et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

A poly-70L/30DL-lactide (PLA70)–β-tricalcium phosphate (β-TCP) composite implant reinforced by continuous PLA-96L/4D-lactide (PLA96) fibers was designed for in vivo spinal fusion. The pilot study was performed with four sheep, using titanium cage implants as controls. The composite implants failed to direct bone growth as desired, whereas the bone contact and the proper integration were evident with controls 6 months after implantation. Therefore, the PLA70/β-TCP composite matrix material was further analyzed in the in vitro experiment by human and ovine adipose stem cells (hASCs and oASCs). The composites proved to be biocompatible as confirmed by live/dead assay. The proliferation rate of oASCs was higher than that of hASCs at all times during the 28 d culture period. Furthermore, the composites had only a minor osteogenic effect on oASCs, whereas the hASC osteogenesis on PLA70/β-TCP composites was evident. In conclusion, the composite implant material can be applied with hASCs for tissue engineering but not be evaluated in vivo with sheep.