Effect of Age and Diabetes on the Response of Mesenchymal Progenitor Cells to Fibrin Matrices
Figure 4
MSC morphology and self-renewal. MSC from an 18-year-old healthy donor were cultured on 10 mg/mL fibrin for 7 days before re-seeding them onto different concentrations of fibrin or TCP. We call these secondary colonies. The different surfaces were again tissue culture plastic, 3 mg/mL fibrin gel, 10 mg/mL, and 30 mg/mL (d). MSC from a 55-year-old donor are cultured on TCP and 30 mg/mL fibrin gel. Colony numbers (a) as well as size (b) are shown and the different morphologies (c–h) are shown. *Denotes a statistically significant difference from the other groups.