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International Journal of Biomaterials
Volume 2012, Article ID 313781, 14 pages
Research Article

A Preliminary Study on the Potential of Manuka Honey and Platelet-Rich Plasma in Wound Healing

1Physical Medicine and Rehabilitation Service, Hunter Holmes McGuire VA Medical Center, Richmond, VA 23249, USA
2Department of Biomedical Engineering, Virginia Commonwealth University, Richmond, VA 23284, USA
3Department of Biology, Virginia Commonwealth University, Richmond, VA 23284, USA
4Department of Nursing, Old Dominion University, Norfolk, VA 23529, USA
5Department of Physical Medicine and Rehabilitation, Virginia Commonwealth University, Richmond, VA 23298, USA

Received 28 June 2012; Accepted 30 October 2012

Academic Editor: Traian V. Chirila

Copyright © 2012 Scott A. Sell et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Aim. The purpose of this study was to determine the in vitro response of cells critical to the wound healing process in culture media supplemented with a lyophilized preparation rich in growth factors (PRGF) and Manuka honey. Materials and Methods. This study utilized cell culture media supplemented with PRGF, as well as whole Manuka honey and the medical-grade Medihoney (MH), a Manuka honey product. The response of human fibroblasts (hDF), macrophages, and endothelial cells (hPMEC) was evaluated, with respect to cell proliferation, chemotaxis, collagen matrix production, and angiogenic potential, when subjected to culture with media containing PRGF, MH, Manuka honey, and a combination of PRGF and MH. Results. All three cell types demonstrated increases in cellular activity in the presence of PRGF, with further increases in activity seen in the presence of PRGF+MH. hDFs proved to be the most positively responsive cells, as they experienced enhanced proliferation, collagen matrix production, and migration into an in vitro wound healing model with the PRGF+MH-supplemented media. Conclusion. This preliminary in vitro study is the first to evaluate the combination of PRGF and Manuka honey, two products with the potential to increase regeneration individually, as a combined product to enhance dermal regeneration.