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International Journal of Biomaterials
Volume 2012, Article ID 319290, 9 pages
Research Article

Effects of a Pseudophysiological Environment on the Elastic and Viscoelastic Properties of Collagen Gels

1Laboratory for Biomaterials and Bioengineering, Department of Mining Metallurgy and Materials Engineering and University Hospital Research Center, Laval University, Quebec City, QC, Canada G1V 0A6
2Laboratory for Biomaterials and Bioengineering, Laval University, Pavillon Pouliot, 1745-E, Québec City, QC, Canada G1K 7P4

Received 9 November 2011; Accepted 2 May 2012

Academic Editor: Bruce Milthorpe

Copyright © 2012 Sébastien Meghezi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Vascular tissue engineering focuses on the replacement of diseased small-diameter blood vessels with a diameter less than 6 mm for which adequate substitutes still do not exist. One approach to vascular tissue engineering is to culture vascular cells on a scaffold in a bioreactor. The bioreactor establishes pseudophysiological conditions for culture (medium culture, 37°C, mechanical stimulation). Collagen gels are widely used as scaffolds for tissue regeneration due to their biological properties; however, they exhibit low mechanical properties. Mechanical characterization of these scaffolds requires establishing the conditions of testing in regard to the conditions set in the bioreactor. The effects of different parameters used during mechanical testing on the collagen gels were evaluated in terms of mechanical and viscoelastic properties. Thus, a factorial experiment was adopted, and three relevant factors were considered: temperature (23°C or 37°C), hydration (aqueous saline solution or air), and mechanical preconditioning (with or without). Statistical analyses showed significant effects of these factors on the mechanical properties which were assessed by tensile tests as well as stress relaxation tests. The last tests provide a more consistent understanding of the gels' viscoelastic properties. Therefore, performing mechanical analyses on hydrogels requires setting an adequate environment in terms of temperature and aqueous saline solution as well as choosing the adequate test.