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International Journal of Biomaterials
Volume 2012, Article ID 397813, 14 pages
http://dx.doi.org/10.1155/2012/397813
Research Article

Oligonucleotide and Parylene Surface Coating of Polystyrene and ePTFE for Improved Endothelial Cell Attachment and Hemocompatibility

1Department of Thoracic, Cardiac and Vascular Surgery, University Hospital, Hoppe-Seyler-Strasse 3, 72076 Tuebingen, Germany
2Fraunhofer Institute for Interfacial Engineering and Biotechnology, Nobelstrasse 12, 70569 Stuttgart, Germany
3Department of Prosthodontics, Section of Medical Materials and Technology, University Hospital, Osianderstrasse 2-8, 72076 Tübingen, Germany
4Inter-University Centre for Medical Technology (IZST), Eberhard Karls University, Silcherstrasse 7, 72076 Tuebingen, Germany
5Institute of Medical Immunology, Charité Universitätsmedizin Berlin, Föhrer Straße 15, 13353 Berlin, Germany
6Berlin-Brandenburg Center for Regenerative Therapies (BCRT), Charité Universitätsmedizin Berlin, Föhrer Straße 15, 13353 Berlin, Germany

Received 15 July 2011; Revised 10 November 2011; Accepted 14 November 2011

Academic Editor: Rolf Larsson

Copyright © 2012 Martina Schleicher et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

In vivo self-endothelialization by endothelial cell adhesion on cardiovascular implants is highly desirable. DNA-oligonucleotides are an intriguing coating material with nonimmunogenic characteristics and the feasibility of easy and rapid chemical fabrication. The objective of this study was the creation of cell adhesive DNA-oligonucleotide coatings on vascular implant surfaces. DNA-oligonucleotides immobilized by adsorption on parylene (poly(monoaminomethyl-para-xylene)) coated polystyrene and ePTFE were resistant to high shear stress (9.5 N/m2) and human blood serum for up to 96 h. Adhesion of murine endothelial progenitor cells, HUVECs and endothelial cells from human adult saphenous veins as well as viability over a period of 14 days of HUVECs on oligonucleotide coated samples under dynamic culture conditions was significantly enhanced (). Oligonucleotide-coated surfaces revealed low thrombogenicity and excellent hemocompatibility after incubation with human blood. These properties suggest the suitability of immobilization of DNA-oligonucleotides for biofunctionalization of blood vessel substitutes for improved in vivo endothelialization.