Optimization of Human Corneal Endothelial Cells for Culture: The Removal of Corneal Stromal Fibroblast Contamination Using Magnetic Cell Separation
Figure 4
Composite fluorescent micrograph images of CMFDA (green) and Na+/K+ATPase pumps (red) of cultured cells established from (a) the “labeled” fraction and (b) the “flow-through” fraction. Cell nuclei were counterstained with DAPI in blue. (Scale bar = 100 μm.)