Optimization of Human Corneal Endothelial Cells for Culture: The Removal of Corneal Stromal Fibroblast Contamination Using Magnetic Cell Separation

Table 2

Efficacy of MACS.

Fraction	CMFDA Positive Cells (%)	Circularity Index
Unsorted Mixture	55.38±7.16	Not Measured
“Labeled”	81.66±3.46	2.61±1.10 *
“Flow-Through”	1.73±1.09	1.42±0.35 *

For the comparison of cell circularity index, significance was achieved between the “labeled” and “flow-through” fraction (𝑧=−9.62^∗𝑃<0.001). The overall sorting efficacy of MACS was calculated as 96.88%  ± 3.50%.