Table 1:
Release and accumulation of
in the culture medium from transwells with CMCA enriched with
.
Days of culture
[
] accumulated in the culture medium in presence of CMCA enriched with 3 mM
[
] accumulated in the culture medium in presence of CMCA enriched with 300
μ
M
[
] accumulated in the culture medium in presence of CMCA enriched with 30
μ
M
[
] accumulated in the culture medium in presence of CMCA enriched with 3
μ
M
Replacement of the old OM with fresh OM
1
1095 ± 33
mM
110 ± 3
M
<3
M
2
1769 ± 68 mM
172 ± 5
μ
M
19 ± 0.5
μ
M
<3
μ
M
3
2185 ± 76 mM
220 ± 6
μ
M
20 ± 0.5
μ
M
<3
μ
M
X
4
256 ± 8
μ
M
24 ± 0.7
μ
M
2.6 ± 0.1
μ
M
<3
μ
M
5
413 ± 8
μ
M
43 ± 1.1
μ
M
4.2 ± 0.1
μ
M
<3
μ
M
6
510 ± 14
μ
M
50 ± 1.6
μ
M
5.1 ± 0.2
μ
M
<3
μ
M
7
570 ± 16
M
59 ± 1.5
M
<3
M
X
8
37 ± 1
μ
M
3.6 ± 0.1
μ
M
<3
μ
M
<3
μ
M
9
59 ± 2
μ
M
6.0 ± 0.2
μ
M
<3
μ
M
<3
μ
M
10
73 ± 2
μ
M
7.2 ± 0.2
μ
M
<3
μ
M
<3
μ
M
X
11
9 ± 0.3
μ
M
<3
μ
M
<3
μ
M
<3
μ
M
12
14 ± 0.4
μ
M
<3
μ
M
<3
μ
M
<3
μ
M
13
17 ± 0.6
μ
M
<3
μ
M
<3
μ
M
<3
μ
M
14
19
± 0.6
M
<3
M
<3
M
<3
M
X
17
<3
μ
M
<3
μ
M
<3
μ
M
<3
μ
M
X
21
<3
μ
M
<3
μ
M
<3
μ
M
<3
μ
M
X
28
<3
M
<3
M
<3
M
<3
M
X
42
<3
M
<3
M
<3
M
<3
M
X
Values expressed as means ± SD of
at times for quantitative ALP and HA analysis are indicated in bold.