Perfusion Flow Enhances Osteogenic Gene Expression and the Infiltration of Osteoblasts and Endothelial Cells into Three-Dimensional Calcium Phosphate Scaffolds

<table class="table-group" id="tab4"><tr><td><table class="table"><tr><td class="thead-hr" colspan="5"><hr/></td></tr><tr><td align="left">Gene</td><td align="center">7-day fold change</td><td align="center"><svg height="10.325" id="M32" style="vertical-align:-0.0pt" version="1.1" viewbox="0 0 11.75 10.325" width="11.75" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,10.325)">
<g transform="translate(72,-63.74)">
<text transform="matrix(1,0,0,-1,-71.95,63.79)">
<tspan style="font-size: 12.50px; " x="0" y="0">𝑃</tspan>
</text>
</g>
</g>
</svg> value</td><td align="center">14-day fold change</td><td align="center"><svg height="10.325" id="M33" style="vertical-align:-0.0pt" version="1.1" viewbox="0 0 11.75 10.325" width="11.75" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,10.325)">
<g transform="translate(72,-63.74)">
<text transform="matrix(1,0,0,-1,-71.95,63.79)">
<tspan style="font-size: 12.50px; " x="0" y="0">𝑃</tspan>
</text>
</g>
</g>
</svg> value</td></tr><tr><td align="left" colspan="5"><hr/></td></tr><tr><td align="left">ALP</td><td align="center"><svg height="12.9125" id="M34" style="vertical-align:-1.95624pt" version="1.1" viewbox="0 0 12.45 12.9125" width="12.45" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,12.9125)">
<g transform="translate(72,-61.67)">
<text transform="matrix(1,0,0,-1,-71.95,63.67)">
<tspan style="font-size: 12.50px; " x="0" y="0">↑</tspan>
</text>
</g>
</g>
</svg>143%</td><td align="center">0.04</td><td align="center">—</td><td align="center">0.19</td></tr><tr><td align="left">OCN</td><td align="center"><svg height="12.9125" id="M35" style="vertical-align:-1.95624pt" version="1.1" viewbox="0 0 12.45 12.9125" width="12.45" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,12.9125)">
<g transform="translate(72,-61.67)">
<text transform="matrix(1,0,0,-1,-71.95,63.67)">
<tspan style="font-size: 12.50px; " x="0" y="0">↑</tspan>
</text>
</g>
</g>
</svg>1072%</td><td align="center">0.07</td><td align="center">—</td><td align="center">0.35</td></tr><tr><td align="left">RUNX-2</td><td align="center">—</td><td align="center">0.73</td><td align="center"><svg height="12.9125" id="M36" style="vertical-align:-1.95624pt" version="1.1" viewbox="0 0 12.45 12.9125" width="12.45" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,12.9125)">
<g transform="translate(72,-61.67)">
<text transform="matrix(1,0,0,-1,-71.95,63.67)">
<tspan style="font-size: 12.50px; " x="0" y="0">↓</tspan>
</text>
</g>
</g>
</svg>55%</td><td align="center">0.01</td></tr><tr class="table-tr"><td colspan="5"><hr class="tbody-hr"/></td></tr></table></td></tr></table>

Changes in mRNA levels with perfusion flow at 7- and 14-day samples (co-cultures).

International Journal of Biomaterials

tab4

Table 4

Table 4: Perfusion Flow Enhances Osteogenic Gene Expression and the Infiltration of Osteoblasts and Endothelial Cells into Three-Dimensional Calcium Phosphate Scaffolds 

Table 4 | Perfusion Flow Enhances Osteogenic Gene Expression and the Infiltration of Osteoblasts and Endothelial Cells into Three-Dimensional Calcium Phosphate Scaffolds 