Figure 5: (a) The release profile of BSA loaded CS NPs at DS : CS ratio of 1 : 1 at pH 7.4, . (b) SDS-PAGE analysis of BSA released from CS NPs: (M) SDS-PAGE standards (BIO-RAD); (A) BSA standard 1 mg/mL; (B) BSA standard 0.2 mg/mL; (C) blank; (D) unloaded CS NPs; (E) and (F) BSA released from CS NPs (DS : CS ratio of 1 : 1) at days 1 and 2.