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International Journal of Biomaterials
Volume 2014, Article ID 383756, 6 pages
http://dx.doi.org/10.1155/2014/383756
Research Article

In Vitro Evaluation of the Antimicrobial Efficacy of Four Endodontic Biomaterials against Enterococcus faecalis, Candida albicans, and Staphylococcus aureus

1Department of Conservative Dentistry & Endodontics, Vydehi Institute of Dental Sciences & Research Centre, No. 82, EPIP Area, Nallurahalli, Whitefield, Bangalore, Karnataka 560066, India
2Department of Microbiology, Vydehi Institute of Medical Sciences & Research Centre, No. 82, EPIP Area, Nallurahalli, Whitefield, Bangalore, Karnataka 560066, India
3Department of Microbiology, St. John’s Medical College, Sarjapur Road, Bangalore, Karnataka 560034, India
4Department of Conservative Dentistry & Endodontics, V. S. Dental College & Research Centre, Bangalore, Karnataka 560001, India
5Department of Conservative Dentistry & Endodontics, Army College of Dental Sciences, Secunderabad 500087, India

Received 31 May 2014; Revised 19 August 2014; Accepted 11 September 2014; Published 12 October 2014

Academic Editor: Sanjukta Deb

Copyright © 2014 Duddi Narendra Nirupama et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Root canal sealers that possess good antimicrobial property can prevent residual and recurrent infection and contribute to successful endodontic therapy. This study evaluated the antimicrobial activity of four endodontic sealers, AH Plus, Tubliseal EWT, EndoRez, and iRoot SP, against three different microorganisms, E. faecalis, C. albicans, and S. aureus, by direct contact test. 10 μL microbial suspensions were allowed to directly contact the four endodontic sealers for 1 hr at 37°C. Subsequently microbial growth was measured spectrophotometrically every 30 min for 18 hours. The microbial suspensions were simultaneously tested to determine the antimicrobial effect of components which are capable of diffusing into the medium. The results revealed that AH Plus and iRootSP had significantly higher antimicrobial activity against E. faecalis. AH Plus and Tubliseal EWT showed significantly higher antimicrobial activity against C. albicans and S. aureus compared to iRoot SP and EndoRez. EndoRez showed the least antimicrobial activity against all the three microorganisms. Inhibition of microbial growth is related to the direct contact of microorganisms with the sealers. In conclusion AH Plus had significantly higher antimicrobial activity against E. faecalis, C. albicans, and S. aureus.