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International Journal of Biomaterials
Volume 2015, Article ID 539494, 6 pages
http://dx.doi.org/10.1155/2015/539494
Research Article

Reduction of Silver Ions by Cell Free Extracts of Westiellopsis sp.

1Department of Bioinformatics, Bharathiar University, Coimbatore 641 046, India
2Phytomatics Laboratory, Centre for Bioinformatics, School of Life Sciences, Pondicherry University, Puducherry 605 014, India
3Plant Molecular Biology Laboratory, Department of Biotechnology, School of Biotechnology and Health Sciences, Karunya University, Coimbatore 641 114, India

Received 19 September 2014; Accepted 21 December 2014

Academic Editor: Bruce Milthorpe

Copyright © 2015 P. T. V. Lakshmi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Biosynthesis of silver nanoparticles using Westiellopsis sp. (A15), a filamentous cyanobacterium belonging to the family Fischerellaceae, has been demonstrated. Aqueous silver ions (Ag+) when exposed to the culture filtrate of Westiellopsis were reduced in the solution, which were characterized by biophysical measures utilizing the UV-Vis spectroscopy, scanning electron microscopy (SEM), and FTIR. The nanoparticles exhibited the maximum absorbance at 420 nm in UV-Vis spectroscopy, while the SEM micrograph revealed that the aggregated nanoparticles vary in size between 20 nm and 5 µm. However, the FTIR analysis provided evidence for presence of proteins in the filtrate to be involved in the reduction of silver ions.