Liposomal Systems as Nanocarriers for the Antiviral Agent Ivermectin

Figure 11

In vitro antiviral effect of ivermectin formulated in liposomes on Huh-7 cells infected with DENV 1. Ivermectin (d) and the following liposomal formulations were tested: #PC3-Ch1-ive0.1 (a), #PC3-Cl1-ive0.1 (b), and #PC3-Br1-ive0.1 (c). In vitro antiviral effect of ivermectin formulated in liposomes on Huh-7 cells infected with DENV 2 (plain lines) and DENV 2 mouse adapted S221 strain (dashed lines). The following liposomal formulations were tested: #PC3-Ch1-ive0.1 (e), #PC3-Cl1-ive0.1 (f), and #PC3-Br1-ive0.1 (g). For comparison, data relative to the free ivermectin are reported in (h). The related empty liposomes do not have any effect on the cells. The phosphatidylcholine concentration (top -axis) is related to empty liposomes while the ivermectin concentration (bottom -axis) is related to ivermectin liposomes. Details on the identification codes and liposomal compositions are included in Table 1. Details on the EC₅₀ values are in Table 3. Data represent the average of 2 determinations ± SD.

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(h)