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International Journal of Biomaterials
Volume 2017, Article ID 5690625, 7 pages
https://doi.org/10.1155/2017/5690625
Research Article

Fabrication of Polycaprolactone/Polyurethane Loading Conjugated Linoleic Acid and Its Antiplatelet Adhesion

1Tissue Engineering and Regenerative Medicine Laboratory, Department of Biomedical Engineering, International University of Vietnam National Universities, Ho Chi Minh City 700000, Vietnam
2Institute of Applied Materials Science, Vietnam Academy of Science and Technology, 01 Mac Dinh Chi, District 1, Ho Chi Minh City, Vietnam
3Graduate University of Science and Technology, Vietnam Academy of Science and Technology, Hanoi, Vietnam

Correspondence should be addressed to Nguyen Thi Hiep; moc.liamg@1891peihtn

Received 20 February 2017; Revised 29 March 2017; Accepted 27 April 2017; Published 16 May 2017

Academic Editor: Kheng-Lim Goh

Copyright © 2017 Ho Hieu Minh et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Polycaprolactone/polyurethane (PCL/PU) fibrous scaffold was loaded with conjugated linoleic acid (CLA) by electrospinning method to improve the hemocompatibility of the polymeric surface. Fourier Transform Infrared Spectroscopy (FT-IR) analysis and Scanning Electron Microscopy (SEM) observation were employed to characterize the chemical structure and the changing morphology of electrospun PCL/PU and PCL/PU loaded with CLA (PCL/PU-CLA) scaffolds. Platelet adhesion and whole blood clot formation tests were used to evaluate the effect of CLA on antithrombotic property of PCL/PU-CLA scaffold. Endothelial cells (EC) were also seeded on the scaffold to examine the difference in the morphology of EC layer and platelet attachment with and without the presence of CLA. SEM results showed that CLA supported the spreading and proliferation of EC and PCL/PU-CLA surface induced lower platelet adhesion as well as attachment of other blood cells compared to the PCL/PU one. These results suggest that electrospinning method can successfully combine the antiplatelet effects of CLA to improve hemocompatibility of PCL/PU scaffolds for applications in artificial blood vessels.