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International Journal of Biomaterials
Volume 2017, Article ID 9034186, 10 pages
https://doi.org/10.1155/2017/9034186
Research Article

Tissue-Engineered Vascular Graft of Small Diameter Based on Electrospun Polylactide Microfibers

1Institute of Macromolecular Compounds, Russian Academy of Sciences, Bolshoy Pr. 31, Saint-Petersburg 199004, Russia
2Peter the Great Saint-Petersburg State Polytechnical University, Polytechnicheskaya Str. 29, Saint-Petersburg 194064, Russia
3Pavlov First Saint-Petersburg State Medical University, Leo Tolstoy Str. 6-8, Saint-Petersburg 197022, Russia

Correspondence should be addressed to P. V. Popryadukhin; ur.liam@np-levap

Received 8 June 2017; Revised 22 August 2017; Accepted 27 September 2017; Published 8 November 2017

Academic Editor: Rosalind Labow

Copyright © 2017 P. V. Popryadukhin et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Tubular vascular grafts 1.1 mm in diameter based on poly(L-lactide) microfibers were obtained by electrospinning. X-ray diffraction and scanning electron microscopy data demonstrated that the samples treated at °C for 1 h in the fixed state on a cylindrical mandrel possessed dense fibrous structure; their degree of crystallinity was approximately 44%. Strength and deformation stability of these samples were higher than those of the native blood vessels; thus, it was possible to use them in tissue engineering as bioresorbable vascular grafts. The experiments on including implantation into rat abdominal aorta demonstrated that the obtained vascular grafts did not cause pathological reactions in the rats; in four weeks, inner side of the grafts became completely covered with endothelial cells, and fibroblasts grew throughout the wall. After exposure for 12 weeks, resorption of PLLA fibers started, and this process was completed in 64 weeks. Resorbed synthetic fibers were replaced by collagen and fibroblasts. At that time, the blood vessel was formed; its neointima and neoadventitia were close to those of the native vessel in structure and composition.