In Vitro Culture of Single Bovine Embryos with Microwell Plates Made of Poly(dimethylsiloxane) Cured under Low Pressure

Figure 1

Images of a PDMS MW plate attached on the bottom of a glass-bottom dish. (a) Schematic diagram showing a PDMS MW plate. Some cylindrical MWs were fabricated on the plate surface. (b) Poly(dimethylsiloxane) microwell (PDMS MW) plate was attached to the bottom of a glass-bottom dish by oxygen plasma treatment. (c) Transmission and (d) median section images of the PDMS MW. The cylindrical MW was 300 μm in diameter and 200 μm in depth. (e) One bovine embryo was introduced into each PDMS MW. Blastocysts (arrow) were observed in the PDMS MW after 7 days of culture. Scale bar: 200 μm.