In Vitro Culture of Single Bovine Embryos with Microwell Plates Made of Poly(dimethylsiloxane) Cured under Low Pressure

<div><b>Characteristics of PDMS plate cured under low pressure.</b> (a) AFM images of the surfaces of regular and LP-PDMS plates. (Left) Regular PDMS plate cured under atmospheric pressure. (Right) LP-PDMS plate cured under low pressure (-0.08 MPa). Scale bar: 1 <i>μ</i>m. (b) Effect of PDMS curing conditions on osmolality of culture medium in regular and LP-PDMS MW. Culture medium added to plastic dish was used as a control. Asterisks were denoted significant differences between regular PDMS MW group (open circle) and other groups (LP-PDMS MW group; open triangle and control; closed diamond) in each Day (P&lt;0.05).</div>

International Journal of Biomaterials

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Figure 2: In Vitro Culture of Single Bovine Embryos with Microwell Plates Made of Poly(dimethylsiloxane) Cured under Low Pressure 