In Vitro Culture of Single Bovine Embryos with Microwell Plates Made of Poly(dimethylsiloxane) Cured under Low Pressure

Table 1

In vitro development of bovine IVF embryos cultured with PDMS MWs¹.

Culture system	No of embryos cultured	No (%) of embryos cleaved^2	No (%) of blastocysts^3
Group culture; (control; 25 embryos/50μl droplet)	75	49 (65)	19 (38)
Single embryo culture; (1 embryo/50μl droplet)	75	53 (71)	0 (0)
WOW; (1 embryo/1 well)	75	50 (67)	11 (22)
Regular-PDMS MW; (1 embryo/ 1 well)	75	53 (71)	3 (6)
LP-PDMS MW; (1 embryo/1 well)	75	56 (75)	18 (32)

experiments were replicated three times.
²The percentage of cultured embryos.
³The percentage of cleaved embryos.
Superscripts in the column indicate significant difference.