Strain and Vibration in Mesenchymal Stem Cells

Figure 4

Effects of uniaxial strain on SM marker expression in MSCs. MSCs were cultured on collagen-coated elastic membranes for 1 day and subjected to 10% uniaxial strain at 1 Hz or kept as static controls for 1 and 2 days. (a) The RNA from each sample was reverse-transcribed into cDNA and the gene expression of SM a-actin, SM-22a, and GAPDH was analyzed by qPCR with their respective primers. The expression level of each gene was normalized with the level of GAPDH in the same sample. The ratio of the gene expression (stretch/static) is presented as mean (±) standard deviation from at least three experiments. Significant difference () from 1. (b) The protein expression of SM a-actin and actin was analyzed by immunoblotting with respective antibody. (c) Statistical analysis of protein expression. The protein expression was quantified, and the expression level of a-actin was normalized with the level of h-actin in the same samples. The ratio of the normalized protein expression (stretch/static) is presented as mean (±) standard deviation from at least three experiments. From Park et al., Biotechnology and Bioengineering [20]. Copyright© 2004 by John Wiley Sons, Inc. Reprinted by permission of John Wiley & Sons, Inc.

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