Coupling of DSPE-PEG-MAL micelles with DTT reduced antibodies was achieved via incubation for twenty-four hours at room temperature. DSPE-PEG-MAL-Ab micelles produced CA-IX directed liposomes by incubation at 60°C with preformed liposomes for two hours.
The MAL group at the terminal end of DSPE-PEG-MAL preformed liposome chains was conjugated to thiolated antibody via incubation overnight with mild shaking at a temperature of 4°C