The N-Terminal Domain of Y-Box Binding Protein-1 Induces Cell Cycle Arrest in G2/M Phase by Binding to Cyclin D1
Figure 3
Fusion proteins containing N-terminal YB-1 sequences are restricted to cytoplasm of the cells. (a) Subcellular localization of the internalized proteins in cells incubated with APYB26GFP (panels B and F), APYB36GFP (Panels C and G), and APYB77GFP (Panels D and H) or APGFP control (A and E) are shown. Representative cells are marked by arrows. In order to show in more detail a representative field (marked by a dashed square) from the 18 hour panels was magnified and shown in a separate box on the right of the figure. (b) Immunoblot analysis of nuclear and cytoplasmic extracts using polyclonal GFP antibody. Note that the three APYBGFP proteins are seen only in cytoplasmic extracts at both time points whereas APGFP was detected in both nuclear and cytoplasmic extracts.
