The N-Terminal Domain of Y-Box Binding Protein-1 Induces Cell Cycle Arrest in G2/M Phase by Binding to Cyclin D1
Figure 6
Evidence that YB-1 interacts with Cyclin D1. (a) Immunoprecipitation of rat hepatoma cell extracts with anticyclin antibodies followed by western blotting using YB-1 antibody (top panel marked A). The blot was stripped and reprobed with cyclin D1 antibody (middle panel marked B). Equal amounts of the extracts were also loaded on the gel, blotted and probed with actin (bottom panel marked C). (b) Indirect immunofluorescence analysis of asynchronous and nocodazole-treated cells. YB-1 antibody (FITC-conjugated) or anticyclin D1 antibody (Texas Red) were used to localize YB-1 and cyclin D1 in asynchronous (panels A to C), double thymidine blocked (panels D to E) or nocodazole-treated (G to I) cells. (c) Immunoprecipitation of fusion proteins isolated from whole cells lysates of asynchronous and G2/M phase synchronized cells.
