International Journal of Cell Biology / 2009 / Article / Fig 6

Research Article

Alteration of Sarcoplasmic Reticulum Release in Skeletal Muscle from Calpain 3-Deficient Mice

Figure 6

Effect of caffeine on [Ca2+]i in isolated myotubes in the presence of Ca2+ channel blockers (Ni2+/Cd2+). Representative traces show the typical time course of the response to 20 mM caffeine observed in (a) wild type (+/+) myotube; (c) calpain 3-deficient (βˆ’/βˆ’) myotube, each treated with Ni2+/Cd2+. The duration of exposure to caffeine (open bars), or 50  πœ‡ M Ni2+/100  πœ‡ M Cd2+ (closed bars) is represented. Bar diagrams (b) and (d) summarize the peak amplitude of the [Ca2+]i response of myotubes to caffeine and CPA. The responses from the wild type myotubes (+/+; b) and calpain 3-deficient myotubes (βˆ’/βˆ’; d), are shown. Fifty  πœ‡ M Ni2+/100  πœ‡ M Cd2+ was added to the extracellular medium prior to the second application. The number of cells tested is given in brackets.

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