Alteration of Sarcoplasmic Reticulum Ca²⁺ Release in Skeletal Muscle from Calpain 3-Deficient Mice

Table 2

Western blot quantification: cleavage of the ryanodine receptor in wild type and calpain 3-deficient skeletal muscles. The gel presented in Figure 1(c) was analysed in terms of band intensities (numbers are given in arbitrary units). The lower band is 58% of the higher band (fixed to 100%) in lane 2, and 51% in lane 3. The amount of the second band is related to small degradation condition (time and temperature), but is always identical in the two situations wild type and calpain 3-deficient skeletal muscles. The blot and the corresponding quantification are representative of at least 3 independent experiments showing no difference between the two types of mice.

Sample	1 = human skeletal muscle	2 = capn3 −/− mouse skeletal muscle	3 = wild type mouse skeletal muscle
Band 1	90982	69472	52878
Band 2	92773	32041	38331
Band 3	49076
Total	232831	101513	91209