Western blot quantification: cleavage of the ryanodine receptor in wild type and calpain 3-deficient skeletal muscles. The gel presented in Figure 1
(c) was analysed in terms of band intensities (numbers are given in arbitrary units). The lower band is 58% of the higher band (fixed to 100%) in lane 2, and 51% in lane 3. The amount of the second band is related to small degradation condition (time and temperature), but is always identical in the two situations wild type and calpain 3-deficient skeletal muscles. The blot and the corresponding quantification are representative of at least 3 independent experiments showing no difference between the two types of mice.