Ret Finger Protein: An E3 Ubiquitin Ligase Juxtaposed to the XY Body in Meiosis
Figure 1
Distribution of RFP isoforms in meiotic and postmeiotic germ cells. (a)–(c) Immunofluorescent labeling of RFP on testis sections corresponding to late VIII and IX stages of mouse spermatogenesis. Sequential optical layers are separated by 4 m from each other. RFP positive signals (green) are detected either as a cap-like structure localized at the border of the nucleus (DAPI, blue) in pachytene spermatocyte (mainly seen in (a)) or as a dot in post meiotic germ cells and in residual bodies (mainly seen in (b) and (c)). Scale bar, 25 m. (d) 20 g of proteins prepared from 80%–90% pure elutriated preparations of pachytene spermatocytes (ps), round spermatids (rs), and elongated spermatids (es) were analyzed by western blotting with rabbit anti-RFP polyclonal antibody. Sizes (kDa) are indicated on the left side and the two RFP isoforms (58 and 68 kDa) are indicated by the arrows. A nonrelevant band was used as loading control (lower part).
