Ret Finger Protein: An E3 Ubiquitin Ligase Juxtaposed to the XY Body in Meiosis
Figure 3
Subcellular localization of RFP and SUMO-I in pachytene spermatocyte nucleus. (a), (b) Overviews of pachytene cells in adult mouse testis (bar, 4 m). XY body (xy) in the nucleus (n) is underlined by the dotted line (c, cytoplasm). The indicated part of the XY body () is enlarged in panels (c) and (d), respectively. (c), (d) RFP and SUMO-I coimmunolabeling detected using gold particles of two different sizes: 15 nm for RFP and 10 nm for SUMO-1 (bar, 160 nm). RFP (r) is significantly concentrated at the border of the nucleus adjacent to the XY body labeled by SUMO-I (s). (e) Nucleus, XY body, and nuclear membrane have been schematized respecting relative size (excepted for nuclear membrane). Mean of beads count is indicated in the corresponding area (nm, nuclear membrane facing the XY body, xy: XY body).
) is enlarged in panels (c) and (d), respectively. (c), (d) RFP and SUMO-I coimmunolabeling detected using gold particles of two different sizes: 15 nm for RFP and 10 nm for SUMO-1 (bar, 160 nm). RFP (r) is significantly concentrated at the border of the nucleus adjacent to the XY body labeled by SUMO-I (s). (e) Nucleus, XY body, and nuclear membrane have been schematized respecting relative size (excepted for nuclear membrane). Mean of beads count is indicated in the corresponding area (nm, nuclear membrane facing the XY body, xy: XY body).
