Immunoneutralization of TGF1 Improves Skeletal Muscle Regeneration: Effects on Myoblast Differentiation and Glycosaminoglycan Content
Figure 7
Effects of anti-TGFβ1 and anti-TβRI antibody treatments on TGF β1 and T βRI protein levels in soleus and EDL derived myoblast cultures. Representative Immunoblots of (a) TGFβ1 or (b) TβRI from cultures of myoblasts derived from soleus and EDL muscles treated with anti-TGFβ1 and anti-TβRI, respectively. Band densities of membranes were scanned. Results are given as bars (white for control and gray for treated cultures) as a percentage of the density is found at day 4 in each condition. Each experiment was performed 3 times and the results were expressed as mean ± SE. Control of loading with -tubulin content in the immunoblot was routinely checked and did not display significant variability upon samples (not shown).
