Role of BMP-4 and Its Signaling Pathways in Cultured Human Melanocytes
Figure 4
BMP-4 reduces the level of total MITF protein. (a) Paired cultures of melanocytes were treated with vehicle or BMP-4 for 24 and 48 hours. At each time point, cells were harvested and immunoblot analysis using monoclonal antibody against MITF was performed. As the loading control, the membrane was immunoblotted for actin. One representative result from three separate experiments is shown. (b) In parallel, total RNA was isolated and quantitative Real-Time PCR was performed using specific primers against MITF-M as described under the Materials and Methods. Paired student t-test was performed for the statistical analysis.
