Research Article

Role of BMP-4 and Its Signaling Pathways in Cultured Human Melanocytes

Figure 6

BMP-4 decreases the levels of 𝑃 𝐾 𝐶 - 𝛽 in mRNA in LH melanoma cells. Quantitative Real-Time PCR was performed using the same cDNA used in semi-quantitative RT-PCR (a). The level of PKC- 𝛽 transcripts were normalized using the level of GAPDH transcripts. Results were then expressed as the percent of the vehicle-treated samples (b). In three independent experiments, BMP-4 significantly decreased the level of PKC- 𝛽 transcripts ( 𝑃 < . 0 3 ).
750482.fig.006a
(a)
750482.fig.006b
(b)