Determinants Present in the Receptor Carboxy Tail Are Responsible for Differences in Subtype-Specific Coupling of -Adrenergic Receptors to Phosphoinositide 3-Kinase
Figure 1
PI-3K activity with β-AR stimulation. HEK 293 cells were
transfected with -AR or -AR. Forty-eight hours after
transfection, cells were stimulated with 1 μM isoproterenol for the indicated
times. PI-3K activity was determined by the level of -PI
produced by the stimulation of -AR () or -AR (■) expressing cells. Top panel
is a representative autoradiograph of TLC separation (). Data from
these experiments is quantitated in the bottom panel as described in Section
2
.
versus
0 minute.