Determinants Present in the Receptor Carboxy Tail Are Responsible for Differences in Subtype-Specific Coupling of -Adrenergic Receptors to Phosphoinositide 3-Kinase
Figure 3
PI-3K Partitioning of PI-3K to the membrane. Forty-eight hours after transfection, cells were stimulated with 1 μM isoproterenol for the indicated
times. Membranes were isolated, solubilized, and approximately 100 μg of
membrane proteins were separated on a 10% SDS-PAGE gel. Membrane-associated
PI-3K levels increased with cells expressing -AR () or -AR (■). Top panel is a representative immunoblot of
the experiments (). Data from
these experiments is quantitated in the bottom panel as described in Section
2
.
versus 0 minute.
