Determinants Present in the Receptor Carboxy Tail Are Responsible for Differences in Subtype-Specific Coupling of -Adrenergic Receptors to Phosphoinositide 3-Kinase
Figure 5
PI-3K activity with chimeric β-AR stimulation. HEK 293 cells were
transfected with
-AR or
-AR. Forty-eight hours after
transfection, cells were stimulated with 1 μM isoproterenol for the indicated
times. PI-3K activity was determined by the level of -PI
produced by the stimulation of
-AR () or
-AR (■) expressing cells. Top panel is a
representative autoradiograph of the TLC separation (). Data from
these experiments is quantitated in the bottom panel as described in Section
2.
versus 0 minute.