Determinants Present in the Receptor Carboxy Tail Are Responsible for Differences in Subtype-Specific Coupling of -Adrenergic Receptors to Phosphoinositide 3-Kinase
Figure 8
p85ΔPI-3K
reduced agonist-induced -AR sequestration. HEK 293 cells were
transfected with p85ΔPI-3K
and the two β-AR subtypes. Cells
were stimulated with 1 μM isoproterenol for 15 minutes (b) and (f). Stimulation
of -AR-transfected cells induced a cluster distribution which is
characteristic of receptor internalization (f). This sequestration was
abolished in presence of either cotransfected p85ΔPI-3K (g) or PTX pretreatment (h). No
significant -AR sequestration was observed under the different conditions used
in these experiments (a)–(d). Representative
images of several experiments () are shown.
