PCI-24781, a Novel Hydroxamic Acid HDAC Inhibitor, Exerts Cytotoxicity and Histone Alterations via Caspase-8 and FADD in Leukemia Cells
The exposure of PCI-24781 increases cytotoxic effects of leukemic cells. Jurkat cells were treated with a range of PCI-24781 doses (0.01 M–10 M), (a) incubated for 24 and 36 hours, and the % cell viability was quantitated by trypan blue exclusion using a Vi-Cell Coulter Counter, (b) incubated for 24 hours, dyed with PI reagent, and analyzed by flow cytometry to assess DNA fragmentation. Error bars represent the means S.D. of three independent experiments. *P < .05 compared to control.