Contribution of Filopodia to Cell Migration: A Mechanical Link between Protrusion and Contraction
Figure 3
Filopodial behavior during continuous cell movement. (a) The formation and movement of filopodial structures was tracked in GFP-T-plastin/mCherry-actin coexpressing cells migrating at different speeds (0.98, 0.71, 0.56, and 0.66 m/min during , , , and , resp.). Only the actin probe is shown. When filopodia started projecting beyond the lamellipodium at , the cell did not start retraction, but instead continued to move forward. Turquoise arrow indicates a stationary filopodium, red arrows represent lateral filopodia. The entire movie can be found in Supplementary Data, Movie 2. (b) Cell protrusion, speed and filopodial behavior. Light blue, red, turquoise and pink column show the speed of nuclear displacement, lamellipodial protrusion, filopodial lateral motion, and the number of stationary and lateral filopodia, respectively. The speed of filopodial lateral motion was measured by the displacement of the distal end of lateral filopodia over time. Scale Bar 10 m.
